CRISPR genome-editing system avoids DNA strand breaks

A variation of the CRISPR/Cas9 genome-editing tool enables more precise manipulation of target genes by not breaking double-stranded DNA and rather reassemble a single point in the targeted DNA sequence. CRISPR gene editing has restructured biomedicine and biotechnology by providing a simple means to engineer genes through targeted double-strand breaks in the genomic DNA of living cells. However, given the random nature of cellular DNA repair mechanisms and the potential for off-target mutations, technologies capable of introducing targeted changes with growing accuracy, such as single-base editors, are preferred. Original Link: