Genetic code growth used to evince biological control mechanism

A team of genomics researchers has expressed the development of a novel biological switching mechanism that authorizes control over the activity of the CRISPR/Cas9 gene-editing tool. CRISPR/Cas9 is considered as the cutting edge of molecular biology technology. Several applications of genome editing by CRISPR-Cas9 necessitate rigid regulation and Cas9 modification have been appropriately generated whose activity responds to small ligands, temperature, or light. To address this issue, investigators at the University of Bath (United Kingdom) and Cardiff University (United Kingdom) speculated that a cheap amino acid such as the lysine derivative, H-Lys(Boc)-OH (BOC), might be harnessed for Cas9 control.
An expanded genetic code is an artificially modified genetic code in which one or more particular codons have been re-allocated to encode an amino acid that is not among the 20 common naturally encoded proteinogenic amino acids. To reveal the principle of Cas9BOC gene editing, the investigators used a transgenic mouse model that carried a gene that caused the animals’ skin to glow green under UV light. Senior author Dr. Anthony Perry, professor of biology and biochemistry at the University of Bath, said, “Our switch is a way of controlling the expression of any protein via genetic code expansion.

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