New tactic appear for malaria parasite tissue culture

A new method for maintaining the liver form of the malaria parasite in tissue culture is expected to lay the foundation for next-generation malaria control and elimination.Malaria liver stages represent an ideal therapeutic target with a bottleneck in parasite load and reduced clinical symptoms;
however, current in vitro liver stage models for Plasmodium vivax and P. falciparum lacks the efficiency necessary for rapid identification and effective evaluation of new vaccines and drugs, especially targeting late liver-stage development and hypnozoites.The investigators developed a 384-well plate culture system using commercially available materials, including cryopreserved primary human hepatocytes and cell culture reagents.
The investigators reported that culture system hepatocyte physiology was maintained for at least 30 days and supported the development of P. vivax hypnozoites and complete maturation of P. vivax and P. falciparum Scizonts. Reducing culture handling to a 384-well microtiter format promoted key morphological and functional characteristics of the native in situ hepatocytes and allowed for high-resolution imaging, seamless image acquisition with faster imaging speed, and integration of automated high-content image analysis.So Approximately “Almost all of the current strategies are focused the blood-stage of malaria – after the person has already become infected,” according to investigator Dr John Adams, “But, in order to eradicate this disease, you need to block the cycle of reinfection and the most efficient way to do that is by blocking the parasite from coming into the person.
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