Synthetic RNAs swell exactness of gene-editing system

Incorporation of next-generation bridged nucleic acids at specific locations in CRISPR-RNAs (crRNAs) broadly reduces off-target DNA cleavage by Cas9 – a major drawback slowing the development of genome editing clinical applications – in vitro and in cells by several orders of magnitude.
The conventional CRISPR/Cas9 system is composed of two parts: the Cas9 enzyme, which cleaves the DNA molecule and specific RNA guides that shepherd the Cas9 protein to the target gene on a DNA strand. In an effort to reduce the degree of off-target cleavage generated by CRISPR-Cas9, investigators at the University of Alberta (Edmonton, Canada) reported that they had used single-molecule fluorescence resonance energy transfer (FRET) experiments to show that BNANC incorporation slowed Cas9 kinetics and improved specificity.

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